protein competition formula

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From:"Jennings, Margaret A." <>
To:'histo' <>
Content-Type:text/plain; charset="iso-8859-1"

I have a monoclonal antibody that I am characterizing and will be using the
protein that the antibody was made to in order to test for competition. I
have a formula that I use when I have peptides to block with. What I need is
an optimal concentration/molar ratio (stochiometry??) for using protein.
Any formula or procedure will be greatly appreciated, otherwise I am
spending my weekend in literature search. 

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