protein competition formula

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From:"Jennings, Margaret A." <jennings@mayo.edu>
To:'histo' <HistoNet@Pathology.swmed.edu>
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I have a monoclonal antibody that I am characterizing and will be using the
protein that the antibody was made to in order to test for competition. I
have a formula that I use when I have peptides to block with. What I need is
an optimal concentration/molar ratio (stochiometry??) for using protein.
Any formula or procedure will be greatly appreciated, otherwise I am
spending my weekend in literature search. 



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