nonspecific staining with ventana

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From:Vicki L McKaughan <>
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We have just purchased the Ventana Nexes Immunostainer.  I am having a problem with heavy
nonspecific staining on NSE and Melanosome.  I
think that it is caused by the Amplifier A&B.
When I remove them from the protocol the stain clears up.  Control tissue stained without the
amplifier works also.  I was wondering if anyone
else has run into this problem and what else I
can try to eliminate it.  

Vicki McKaughan HT (ASCP)

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