Yeast vs. bacteria

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From:Bret Morrow <>
To:HistoNet Server <>
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<!doctype html public "-//w3c//dtd html 4.0 transitional//en"> <html> Greetings, <p>I am interested in staining yeast cells in solution in order to 1) determined concentration & total # and 2) test for possible contamination with bacteria, most commanly lactobacteria.  The "sample" is free-floating yeast, <b>not</b> in any tissue.  OK, OK it's a starter for my home brew! <p>I can handle the quantification end, but 1) what can I use to stain the yeast and 2) can I determine if its contaminated from the same stain?  I have been told to use methylene blue, but the concentration and other details (eg in what?, at what pH?, using what staining schedual?) are missing.  Any help would be appriciated <p>TIA <br>____________________________________________ <br>Bret A. Morrow, Ph.D. <br>Associate research scientist <br>Associate clinical professor <br>Yale University School of Medicine <br>New Haven, CT 06520-8066 <p>voice 203.785.4515 <br>fax  203.785.7670 <br>email <br> </html>
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