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<p>I am interested in staining yeast cells in solution in order to 1) determined
concentration & total # and 2) test for possible contamination with
bacteria, most commanly lactobacteria. The "sample" is free-floating
yeast, <b>not</b> in any tissue. OK, OK it's a starter for my home
<p>I can handle the quantification end, but 1) what can I use to stain
the yeast and 2) can I determine if its contaminated from the same stain?
I have been told to use methylene blue, but the concentration and other
details (eg in what?, at what pH?, using what staining schedual?) are missing.
Any help would be appriciated
<br>Bret A. Morrow, Ph.D.
<br>Associate research scientist
<br>Associate clinical professor
<br>Yale University School of Medicine
<br>New Haven, CT 06520-8066