Re: nonspecific staining with ventana

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From:Victoria Baker <>
To:Vicki L McKaughan <>
Content-Type:text/plain; charset=us-ascii

Hi Vicki,

I didn't experience this with the NexES, but I did
have some of the same problems with the ES. 
Amplification isn't necessary with NSE or Melanosome
(I've had experience with both Dako & Ventana
clones/antibodies).  I did use a 3% H2O2 in METOH
block for 10 minutes prior to starting the program
though. The NSE is especially prone to non-specific
staining in the control because pancreas autolyzes
(did I spell that right?  It's after 10pm and my brain
is ready for bed) and that picks up like iron filing
to a magnet.  

Good luck.


Vikki Baker, HT (ASCP)
American Health Foundation
Valhalla, New York  

--- Vicki L McKaughan <> wrote:
> We have just purchased the Ventana Nexes
> Immunostainer.  I am having a problem with heavy
> nonspecific staining on NSE and Melanosome.  I
> think that it is caused by the Amplifier A&B.
> When I remove them from the protocol the stain
> clears up.  Control tissue stained without the
> amplifier works also.  I was wondering if anyone
> else has run into this problem and what else I
> can try to eliminate it.  
> Thanks,
> Vicki McKaughan HT (ASCP)
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> addresses.
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