Hi Vicki,

I didn't experience this with the NexES, but I did
have some of the same problems with the ES. 
Amplification isn't necessary with NSE or Melanosome
(I've had experience with both Dako & Ventana
clones/antibodies).  I did use a 3% H2O2 in METOH
block for 10 minutes prior to starting the program
though. The NSE is especially prone to non-specific
staining in the control because pancreas autolyzes
(did I spell that right?  It's after 10pm and my brain
is ready for bed) and that picks up like iron filing
to a magnet.  

Good luck.

Sincerely,

Vikki Baker, HT (ASCP)
American Health Foundation
Valhalla, New York  



--- Vicki L McKaughan <vlm_23@n2mail.com> wrote:
> We have just purchased the Ventana Nexes
> Immunostainer.  I am having a problem with heavy
> nonspecific staining on NSE and Melanosome.  I
> think that it is caused by the Amplifier A&B.
> When I remove them from the protocol the stain
> clears up.  Control tissue stained without the
> amplifier works also.  I was wondering if anyone
> else has run into this problem and what else I
> can try to eliminate it.  
> 
> Thanks,
> Vicki McKaughan HT (ASCP)
> 
> 
> 
> What are you N2?  Choose from 150 free e-mail
> addresses.
> http://www.n2mail.com
> 
> 
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com