Re: melanin/DAB/AEC and another

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From:Robert Schoonhoven <>
To:Gayle Callis <>
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While I haven't used the NovaRed I do use either Dako's or Biogenix's
New Fuchsin and clear with Richard Allan's Clearite 3.  This gives a
beautiful crisp red end product which is very permanent.  I've been
using this for over 8 years now and when going back to the old slides
they look just as good as slides stained  last week.  My Image Analysis
system even agrees with me.:-)  I also do a lot of DAB but I just use
xylene on those.

Gayle Callis wrote:
> Vector now makes a very sensitive chromogen, NovaRed as a permanent
> replacement for AEC, could work for contrast with melanin, and is as
> sensitive as DAB, if you wanted to stay with the HRP system.  It does not
> require aqueous based mounting media.  Could be worth a try
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
> 406 994-4705
> 406 994-4303

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

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