Kimberly;

Anti-vimentin would not be a good choice to stain with since is does cross react
with mouse and rat vimentin.  And the secondary reagents would also cause
background in the mouse portion of the tissue.You might want to try a specific CD
marker or relavent marker  for the particular type of tumor that is growing.  (eg;
b-cell lymphoma you might try a HRP conjugated-CD20 since most CD markers do not
have close homolgy to mouse/rodent.
Barb:)

Kimberly L Merriam wrote:

> Corazon,
>
> Try staining the tumors with anti-vimentin, it should stain the human cells and
> not the mouse cells.  There are lots of vendors that make this antibody.  We
> use DAKO's V9 clone.
>
> Kim Merriam
> TKT
> Cambridge, MA
>
> Corazon Bucana wrote:
>
> > Just curious if anyone has a good antibody to distinguish human tumor cells
> > growing in nude mice. We have an investigator who is interested in tracking
> > micrometastasis of human tumors growing in nude mice by immunohistochemistry
> > .  Use of exotic markers such as GFP will be the last resort.  I tried an
> > antibody to class I but some tumors are known to lose this antigen.  I would
> > appreciate any comments or suggestions.
> >
> > C. Bucana
> > *******************************************************
> > Corazon D. Bucana, Ph.D.
> > Department of Cancer Biology
> > U.T. M.D. Anderson Cancer Center
> > 1515 Holcombe Blvd.  Box 173
> > Houston, Texas 77030
> > Phone: (713) 792-8106
> > FAX: (713) 792-8747
> > Email:bucana@audumla.mdacc.tmc.edu
> > FAX: (713) 792-8747