Hi,
    An altogether too familiar question ... What is the tissue fixed in?? Also
how are you antigen retrieving them. For this we use A steamer and DAKO's
epitope retrieval solution.
Amos Brooks

Pathrm35@cs.com wrote:

> Fellow Histonetters,
>    I am having problems with CD3 and Ki-67 immunos staining correctly. I am
> using
> Biogenex antibodies and have tried their procedure by hand and on their
> immunostainer.
>    I have had mixed results with the Ki-67 and no results with the CD3, both
> on the stainer and by hand. If anyone has any suggestions I greatly
> appreciate it.
>
>    Ron Martin, B.Sc. HTL,HT (ASCP)
>    Palm Beach Pathology
>    West Palm Beach, Fl