Re: dehydrated brain...
<< Previous Message | Next Message >>
From: | FlaHistoTek@aol.com |
To: | pmarcum@polysciences.com, Aidan.Schurr@hvh.co.nz, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
In a message dated 2/22/00 9:32:34 AM Eastern Standard Time,
pmarcum@polysciences.com writes:
<< nfortunately, once the complete dehydration is done there is very little
you can do. If it was NBF there is even a good chance you have precipitated
buffer salts all over the outside as a crust. This will further inhibit any
penetration of fluids. Maybe someone else can add more. Pam
-----Original Message-----
From: a i d a n s c h u r r [mailto:Aidan.Schurr@hvh.co.nz]
Sent: Monday, February 21, 2000 11:10 PM
To: histonet@pathology.swmed.edu
Subject: dehydrated brain...
Hi all,
An interesting question / problem...
We have a brain which was fixed approx. 2 years ago in NBF, then
stored. Unfortunately the container was not sealed, and the NBF
evaporated. We now have an extremely dehydrated and shrivelled
brain, and a consultant who wants histology on it!! Any ideas as to
rehydration / salvage would be greatly appreciated.
Cheers,
Aidan Schurr, New Zealand
___________________________________________________
a i d a n c s c h u r r
mlso, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
___________________________________________________
>>I have had limited experience with small specimens left out all night,
and had to be rehydrated. When setting processor teck dropped block then we
had to hunt for it. When found we would use a glycerin solution in large
container, with constant stirring. The brain, being so large, I wouldnt know
where to begin. I do know the glycerin works on rehydrating tissue. Hope this
helps a bit. Nothing to lose.
Barb Stone
<< Previous Message | Next Message >>