Re: any ideas?

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From:Tony Henwood <>, Alan Flechtner <>
Content-Type:text/plain; charset=US-ASCII

Dear Alan

> Hi all! Recently, our lab has been experiencing a hazy, lack of detail at
> high magnification on all of our H&E's and special stains. I've changed ALL
> reagents, cover slips, slides and mounting media yet still no change in
> results. Could it be the formalin? We prepare 55 gallons of 10% NBF at a
> time and the problems started around the same time a new batch of formalin
> was made. Any ideas would be greatly appreciated. Thanks.

Have you tried checking the cleanliness, or lack of, of your high 
magnification objective. Try cleaning them, check you Kohler 
illumination and see what happens (Some one is always spraying oil on 
my 40X objective and it really cheeses me off!!!)

Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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