Re: Timm's Method

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From:"J. A. Kiernan" <>
To:Tina Cardamone <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Mon, 28 Feb 2000, Tina Cardamone wrote:

> I have formalin fixed paraffin embeded tissue and I am concerned that fresh 
> tissue is required for this stain!!!!

  Yes that's right. The zinc (in hippocampus) is precipitated
  with H2S or Na2S before fixation. A physical developer then
  amplifies the sites of deposition of ZnS.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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