Re: Timm's Method
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Tina Cardamone <kcard@myriad.its.unimelb.edu.au> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Mon, 28 Feb 2000, Tina Cardamone wrote:
> I have formalin fixed paraffin embeded tissue and I am concerned that fresh
> tissue is required for this stain!!!!
Yes that's right. The zinc (in hippocampus) is precipitated
with H2S or Na2S before fixation. A physical developer then
amplifies the sites of deposition of ZnS.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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