Re: Rat teste processing
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From: | Geoff McAuliffe <mcauliff@UMDNJ.EDU> |
To: | "RODRIGUEZ, RICHARD" <RICHARD.RODRIGUEZ@spcorp.com> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
"RODRIGUEZ, RICHARD" wrote:
> We have a question on the processing of rat testes fixed in Bouin's.
> If anyone else out there is performing this type of procedure please
> let us know how you handle the following:
> 1. How do you rinse the Bouin's (picric acid) out of the tissue?
I have always used 4 changes of 50% ethanol, 1-1.5 hours each, to remove
excess Picric acid from tissues. Then proceed to the remainder of dehydration
as usual.
> 2. Do you use alcohol rinses and end in 70% alcohol?
> 3. Do you perform these rinses manually or on a tissue processor?
Maually.
> 4. If a processor is used, which one?
>
> We are currently manually rinsing the testes in alcohol down to 70%
> where they remain until they are processed to block. The idea of
> using a tissue processor to perform this task seems good but Sakura
> has informed us that they do not recommend doing this because the
> picric acid will damage the VIP processor.
>
> Any suggestions, ideas let us know.
>
> Thanks
>
> Richard Rodriguez
> Schering-Plough Research Institute
> Lafayette NJ
> 973-579-4282
Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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