Re: IHC staining and animal tissue

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From:Katri Tuomala <>
To:mbpohl <>
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Your mouse tissues have gone through quite a fixation mixture. First B5
(mercury fixative), then 10% NBF and finally a zinc formalin. I'm not
supprised that they are dried out and personally I would not guarantee
any immuno results on these tissues. I am sorry to be so negative, but
how can you control your procedure? I hope somebody will come to your
aid, I'd chuck them all.


Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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