Re: IHC and Melanin Pigment
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From: | amos brooks <atbrooks@snet.net> |
To: | Mike or Carol Schreiber <mjsch@pro-ns.net> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi,
The main problem we ran into with oxidizing the melanin with Potassium
Permanganate (KMnO4 for short <-- yes I am that lazy) and Oxalic acid or with
Hydrogen Peroxide was that the sections fell off frequently. If you do this use
a dilute KMnO4, 0.5% at most. I have read an article about Azure B and know of a
lab that uses it. I could get more information on it for you if you want. It
seems to work for them.
Amos Brooks
Mike or Carol Schreiber wrote:
> Dear Histonetters,
> How is anybody dealing with heavy deposits of Melanin pigment in (skin)
> tissue that is to be stained for HMB-45 and/or S-100? We are using DAKO's
> Envision+/DAB+ detection system, on the DAKO autostainer. Will a Melanin
> Bleach procedure using Potassium Permanganate and Oxalic Acid adversely
> affect subsequent immunostaining? I read something about using Azure-B as a
> counterstain instead of Hematoxylin, which is supposed to stain the melanin
> a green-blue thus differentiating it from the brown staining of the DAB
> chromogen. Has anybody tried this? Thanks for any help you can give me with
> this problem.
>
> Mike Schreiber HT(ASCP)
> Three Rivers Pathology
> Roseville, Minnesota
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