Re: IHC and Melanin Pigment
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From: | Katri Tuomala <katri@istar.ca> |
To: | Mike or Carol Schreiber <mjsch@pro-ns.net> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi Mike,
The potassium permanganate bleach will destroy the antigenicity. I use
AEC instead of DAB as the chromogen for those cases with any endogenous
brown pigment.
AEC produces a red end product, which is easily distinguishable from the
melanin pigment. You'll just have to use an aqueous mounting media.
Katri
Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada
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