Hi,
    Are you having this contamination on your other slides too? If not then
there is not much you can do except tell the cytology department about the
problem since this is where the problem must be coming from.
    As a suggestion of the possible source of the problem, the specimen chambers
that are used to make the cytospins may be being reused and not completely
washed out. If this is the case then they should use disposable holders in spite
of the lack of cost effectiveness. It beats a misdiagnosis.
Amos Brooks

"Scott, Allison D" wrote:

> We are having  a problem with a contaminate fungus that is being picked up
> with the gms stain on cytospins.  The patient slide is contaimated with a
> fungus.  We use sterile water for our water rinses, and the stock and
> working solutions are made up with sterile water also.  We don't make the
> cytospins.  Th cytology person makes them.  This happened with a case last
> week and also this week.  The other cases that the gms was done on did not
> have anything else staining that was not supose to.  These were case that we
> cut.  The water baths have sterile water also.  If anyone has a probable
> solution for this problem, that we have not considered please reply.
> Allison Scott