Re: GMA staining: Technovit v. JB-4
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From: | Tony Henwood <henwood@mail.one.net.au> |
To: | Histonet@pathology.swmed.edu, Carla_Aiwohi@usgs.gov (Carla Aiwohi) |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Dear Carla,
>
> Recently our lab began using Technovit 7100 glycol methacrylate instead of
> JB-4. We have been very happy with the results, however staining (H&E, Giemsa)
> is rather pale - photos are usually too light to submit for publication.
>
> Increasing staining time (in Gill's #3 hematoxylin and alcoholic eosin) and
> section thickness (to 3 microns) has helped a little, but the staining isn't as
> dark as with JB-4. We switched from JB-4 because of the 'cracked paint'
> artifact in areas without tissue. These were also referred to as mini-folds in
> J of Histotechnology 19(4):297-311.
>
> We want to continue using Technovit so does anyone have any suggestions on how
> to increase staining quality in these sections? Also, has anyone else
> experienced the 'cracked paint' phenomenon and how did you correct it?
Try a Celestine Blue/Haematoxylin or an Iron Haematoxylin. I have
found that they are an improvement.
You may also consider using a "hard" fixative such as Bouins, Hellys
(containing Hg or Zn) or even FAA.
Hope this helps,
Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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