Re: Formaldehyde, Authority, and Re: FLT-1/FLK-1 ISH
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From: | oshel@terracom.net (Philip Oshel) |
To: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
John,
Well, yes, but this ignores the MeOH in formaldehyde, and it's because of
the MeOH that us electron types use paraformaldehyde. (There, I condemned
myself. I do do LM also, honest!)
And I made the mistake of trying to fix cultured macrophages with 10% NBF.
Distorted them horribly, because of the methanol.
Phil
>> I thought there was little or no difference between 4% paraformaldehyde and
>> 10% neutral buffered formalin. Will a chemist step in and resolve my
>> confusion??
>
> I'm not a chemist, but know enough to assure you that you
> are entirely correct in your thoughts about formaldehyde.
>
> All this has been the subject of dozens of HistoNet
> communications. Now the archives are again available
> on the web, there's no point re-quoting the textbooks
> yet another time.
>
> The Archives are very good - well indexed etc - but
> we must remember that this informal exchange of
> questions and answers is not the font of eternal
> wisdom. The only reliable sources of information are
> peer-reviewed journals and textbooks (which cite the
> journals, and also are quite severely vetted by their
> publishers before being taken on as commercial risks).
>
> Every lab doing histology should have a shelf full of
> big textbooks (10 X $100 = $1000) and two or three
> journals (@ $100 to 200 per year each: Max. $600 per
> year). Total expenditure is $1000 statim and a few
> hundred a year thereafter. This is well below 1% of
> the salary of some worthless senior administrator ...
> (Perhaps I'd better say no more, having made the point.)
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
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