Re: Fixing gelatin blocks
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Peter Bannister <peterbann@yahoo.com> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Fri, 18 Feb 2000, Peter Bannister wrote:
> I hope someone can help me. Does anyone know how to
> fix or otherwise harden gelatin without the use of
> glutaraldehyde?
The traditional way to harden gelatin is to immerse
the block in 4% formaldehyde overnight. Do it at
fridge temp if the gelatin is dilute enough to go
sloppy at room temperature. This gives nice square
frozen sections that are easy to handle. The gelatin
cannot be removed after it's been fixed in this way.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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