Re: Fixing gelatin blocks

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From:"J. A. Kiernan" <>
To:Peter Bannister <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Fri, 18 Feb 2000, Peter Bannister wrote:

> I hope someone can help me. Does anyone know how to
> fix or otherwise harden gelatin without the use of
> glutaraldehyde?

  The traditional way to harden gelatin is to immerse
  the block in 4% formaldehyde overnight. Do it at
  fridge temp if the gelatin is dilute enough to go
  sloppy at room temperature. This gives nice square
  frozen sections that are easy to handle. The gelatin
  cannot be removed after it's been fixed in this way.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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