Re: Citrate Buffer pH10
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Gino De Marchi <ddk.mi@iol.it> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Sun, 27 Feb 2000, Gino De Marchi wrote:
> Does anybody have a good recipe for citrate buffer pH 10!
For pH 10.0:
Temperature
20C 50C
2M NaOH 3.59 ml 3.49 ml
2M citric acid 10 ml 10 ml
Dilute to 100 ml with water.
Source: Table 10.9 in Perrin & Dempsey "Buffers for
pH and Metal Ion Control. London: Chapman & Hall,
1974. p. 130. References are given there for the primary
source and for buffers at temperatures up to 91C.
(Note that ordinary citric acid-sodium citrate buffer
if effective only in the range 3.0-6.2.)
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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