Re: Citrate Buffer pH10

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From:"J. A. Kiernan" <>
To:Gino De Marchi <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Sun, 27 Feb 2000, Gino De Marchi wrote:

> Does anybody have a good recipe for citrate buffer pH 10!

For pH 10.0:
                      20C          50C
    2M NaOH          3.59 ml      3.49 ml
    2M citric acid  10 ml        10 ml
  Dilute to 100 ml with water.

  Source: Table 10.9 in Perrin & Dempsey "Buffers for
    pH and Metal Ion Control. London: Chapman & Hall, 
    1974. p. 130. References are given there for the primary
    source and for buffers at temperatures up to 91C.
    (Note that ordinary citric acid-sodium citrate buffer
     if effective only in the range 3.0-6.2.)

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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