Re: Azure B; black DAB

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From:Tony Henwood <>
To:"J. A. Kiernan" <>
Content-Type:text/plain; charset=US-ASCII

Dear John,

Wouldn't the melanin also reduce the silver, since it argentaffilic?

> On Thu, 24 Feb 2000, Forney Constance M Civ 74 MDSS/SGSC wrote:
> > Also - my pathologist wants his DAB to stain black instead of brown
> > - anyone have a procedure for that ?
>   This is done by including cobalt or nickel ions in the 
>   DAB-containing incubation medium. Any textbook should 
>   have a recipe. The colour of the oxidation product is 
>   blue-black instead of brown. There are also silver
>   methods for amplifying the brown product, but these are
>   quite complicated procedures.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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