Re: Azure B; black DAB
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Tony Henwood <henwood@mail.one.net.au> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Mon, 28 Feb 2000, Tony Henwood wrote:
> Wouldn't the melanin also reduce the silver, since it argentaffilic?
> > .... There are also silver
> > methods for amplifying the brown product, but these are
> > quite complicated procedures.
A good point Tony. The argentaffin reaction of melanin is with
ammoniacal silver solutions, which are quite strongly alkaline.
The amplification of DAB is done with a physical developer
of the Gallyas type, at somewhat less alkaline pH. My notes
on 3 papers (1986 to 1998) don't mention melanin. Perhaps the
authors didn't try their methods on skin or substantia nigra.
I would guess that you are right, and melanin might well
get co-amplified along with DAB in the silver enhancement
methods. Perhaps a message will come in from someone who
has tried the method on melanin-containing material.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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