Re: Azure B; black DAB
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Forney Constance M Civ 74 MDSS/SGSC <Constance.Forney@wpafb.af.mil> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Thu, 24 Feb 2000, Forney Constance M Civ 74 MDSS/SGSC wrote:
> Also - my pathologist wants his DAB to stain black instead of brown
> - anyone have a procedure for that ?
This is done by including cobalt or nickel ions in the
DAB-containing incubation medium. Any textbook should
have a recipe. The colour of the oxidation product is
blue-black instead of brown. There are also silver
methods for amplifying the brown product, but these are
quite complicated procedures.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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