Re: Autostainer for IHC

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From:Robert Schoonhoven <rschoonh@sph.unc.edu>
To:"Horn, Hazel" <HornHazelV@exchange.ach.uams.edu>
Reply-To:
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I agree with Hazel on this one as I have seen on campus demos of both. 
I would also note that in my case it doesn't matter if the MACHINE takes
1 hour or 4 hours to complete a run.  That is 1 to 4 hours where I can
be doing something more productive (4 hours = 1 round of golf).

"Horn, Hazel" wrote:
> 
> I take an exception with Donald's answer.    The Dako stainer can complete a
> run in as little as 45 minutes.   I hardly think the NEXes can do one in 23
> minutes.     With the autostainer you can use your own antibodies from
> whomever you choose.     You can run multiple programs at the same time.
> i.e.
> titration runs,  patient slides, etc.     You should consider Mr. Hill's
> response as a sales pitch.   After all,
> he works for  Ventanna.
> Hazel
> 
> > -----Original Message-----
> > From: Donald Hill [SMTP:sales101@bellsouth.net]
> > Sent: Sunday, February 20, 2000 11:15 PM
> > To:   Sharon Osborn; perl
> > Cc:   Histonet@pathology.swmed.edu
> > Subject:      Re: Autostainer for IHC
> >
> > I think you should look at the NEXes, although DAKO does 48 slides, it can
> > take 2 to three times as long to run those slides, I've seen head to head
> > comparisons between the TAT and I would have to ask, why would you want to
> > wait twice as long to get your slides? Also with the set up times
> > involved,
> > Ventana's system is so much faster and easier to set up.
> >
> > I've seen people on here talking about the detection being closed and
> > possibly costing more, Ventana is the only company that can gaurantee
> > slide
> > cost using thier reagents due to the 100ul per slide gaurantee and by
> > using
> > thier optimized reagents, high quality, consistant slides is what you get
> > day in and day out. If cost is the most important factor in considering
> > autostaining systems, Ventana by far is one of the most cost effective
> > systems on the market. Give them a call and I am sure they will prove it
> > to
> > you as they did to me.
> >
> > That's why I am working for them now!
> >
> >
> > -----Original Message-----
> > From: Sharon Osborn <topper2u@earthlink.net>
> > To: perl <perl@agri.huji.ac.il>
> > Cc: Histonet@pathology.swmed.edu <Histonet@pathology.swmed.edu>
> > Date: Sunday, February 20, 2000 10:24 PM
> > Subject: Re: Autostainer for IHC
> >
> >
> > >Dr. Perl,  The DAKO will do 48 slides, the Leica (if same as the USA
> > model)
> > >does 24 slides (I think).  It could be suitable for small volumes.  Here,
> > >Zymed markets it with their detection system.  sharon osborn
> > >
> > >perl wrote:
> > >
> > >> Dear Histonetters,
> > >>
> > >> We are in the process of purchasing an Automated
> > >> immunohistochemical stainer.
> > >> If anyone has experience in this field, please let me know which
> > >> system would you recommend.
> > >> Here we can by machines made by: Ventana, Lab Vision, Lleica.
> > >>
> > >> Samuel
> > >> Dr Samuel Perl
> > >> Head, Dept. of Pathology
> > >> Kimron Veterinary Institute
> > >> P.O.Box 12, 50250 Bet Dagan
> > >> ISRAEL
> > >> E-mail: perl@agri.huji.ac.il  Fax: (972)39681730  Phone: (972) 39681635
> > >
> > >
> > >
> >

-- 
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone 
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910)



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