Re: Autostainer for IHC

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From:Luis Chiriboga <Luis.Chiriboga@med.nyu.edu>
To:mbpohl <mbpohl@acsu.buffalo.edu>
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Nexus does not allow you to run different procedures on the same run
(Titration, paraffin, Non-paraffin).Closed architecture does not allow
you to manipulate multiple variables.  You can turn the heat off on the
nexus.  Reagent issues are the same on the nexus

Luis Chiriboga

mbpohl wrote:
> 
> We have had the Ventana ES since July 1996.  Reliable, easy to use, minimum
> maintenance.  Down side:  Can only run at 37 degrees C;  limited to use
> their detection kits - and when the price goes up, you feel helpless
> (unless you have a reasonable sales person);  in the past little support
> from sales rep's --> hopefully I am seeing an improvement there;  limited
> technical help -- again, I am told I will see an improvement there;  you
> either have to just use antibody dispensers for a run OR you can manually
> titrate your own dilutions applying them with a pipette - not both.  I am
> told the Ventana Nexus allows both ways to apply antibodies during the same
> run - dispenser and manually titrating them.
> 
> I will be interested in hearing from others.
> perl wrote:
> 
> > Dear Histonetters,
> >
> > We are in the process of purchasing an Automated
> > immunohistochemical stainer.
> > If anyone has experience in this field, please let me know which
> > system would you recommend.
> > Here we can by machines made by: Ventana, Lab Vision, Lleica.
> >
> > Samuel
> > Dr Samuel Perl
> > Head, Dept. of Pathology
> > Kimron Veterinary Institute
> > P.O.Box 12, 50250 Bet Dagan
> > ISRAEL
> > E-mail: perl@agri.huji.ac.il  Fax: (972)39681730  Phone: (972) 39681635



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