Hi Taffy,

I found the article "Detection of Apoptosis by a Modified Trichrome
Technique," in the Dec. 1999, Journal of Histotechnology, vol. 22, No.4.
It over stained my bone sections in parapffin badly so I did
further modification and ended up with the staining procedure below:

3 seconds in 	Acid fuchsin	0.50 gm
		Orange G	0.025 gm
		Glacial acetic	1 ml	
		acid
		Distilled H20	up to 100 ml

Rinse in DH20

1 minute	5% phosphotungstic acid in DH20

Rinse in DH20

3 minutes in 	Light Green	.50 gm
		Glacial acetic	1 ml
		acid
		Distilled H20	up to 100 ml
Rinse in DH20

Brief rinse in 	100% ETOH

3 changes	Clearing Agent

I have not done any specimens in plastic, but this works well for the
paraffin sections.  Hope it works for you.

Trisha Emry
Orthodontics Research Lab
U of WA, Seattle

On Fri, 18 Feb 2000, Taffy Hooser wrote:

> Hello Histonetters,
> 
> I am looking for a procedure on Apoptosis on Bone sections in paraffin and
> MMA.  If anyone has ANY information on the subject, I know our post-docs
> would be MOST happy!!
> Thanks in advance for your help.
> Taffy Hooser
> hooser@anatomy.iupui.edu
> 
> 
> 

Trisha