Re:

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From:DDittus787@aol.com
To:louiset@mail.saimr.wits.ac.za, histonet@pathology.swmed.edu
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Fresh frozen tissue is notorious for high background , it is my experience 
that if you shorten incubation time , you will get less background., and 
fresh tissue seems to need less time on all reagents, simply because it is 
fresh.
                                                         Dana



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