We have the same problem with EGFr when using the amplified detection system
on the Nexes.
Patsy

-----Original Message-----
From: Vicki L McKaughan [mailto:vlm_23@n2mail.com]
Sent: Tuesday, February 15, 2000 1:18 PM
To: histonet@pathology.swmed.edu
Subject: nonspecific staining with ventana


We have just purchased the Ventana Nexes Immunostainer.  I am having a
problem with heavy
nonspecific staining on NSE and Melanosome.  I
think that it is caused by the Amplifier A&B.
When I remove them from the protocol the stain clears up.  Control tissue
stained without the
amplifier works also.  I was wondering if anyone
else has run into this problem and what else I
can try to eliminate it.  

Thanks,
Vicki McKaughan HT (ASCP)



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