RE: dehydrated brain...
<< Previous Message | Next Message >>
From: | "MacDonald, Jennifer" <jmacdonald@sach.org> |
To: | a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz>, histonet@pathology.swmed.edu, 'Pam Marcum' <pmarcum@polysciences.com> |
Reply-To: | |
Content-Type: | text/plain |
Because you really have nothing to lose I would rehydrate the brain in
normal saline. I really don't know how long would be a good amount of time.
It is dependant on the size of the tissue and the extent of the dehydration.
Once the brain is sufficiently rehydrated proceed as usual. I must add that
I have not done this.
Jennifer MacDonald
San Antonio Community Hospital
Upland, CA 91786
(909) 985-2811 ext. 4148
jmacdonald@sach.org
> ----------
> From: Pam Marcum[SMTP:pmarcum@polysciences.com]
> Sent: Tuesday, February 22, 2000 8:02 AM
> To: a i d a n s c h u r r; histonet@pathology.swmed.edu
> Subject: RE: dehydrated brain...
>
> Unfortunately, once the complete dehydration is done there is very little
> you can do. If it was NBF there is even a good chance you have
> precipitated
> buffer salts all over the outside as a crust. This will further inhibit
> any
> penetration of fluids. Maybe someone else can add more. Pam
>
> -----Original Message-----
> From: a i d a n s c h u r r [mailto:Aidan.Schurr@hvh.co.nz]
> Sent: Monday, February 21, 2000 11:10 PM
> To: histonet@pathology.swmed.edu
> Subject: dehydrated brain...
>
>
> Hi all,
>
> An interesting question / problem...
>
> We have a brain which was fixed approx. 2 years ago in NBF, then
> stored. Unfortunately the container was not sealed, and the NBF
> evaporated. We now have an extremely dehydrated and shrivelled
> brain, and a consultant who wants histology on it!! Any ideas as to
> rehydration / salvage would be greatly appreciated.
>
> Cheers,
> Aidan Schurr, New Zealand
>
> ___________________________________________________
> a i d a n c s c h u r r
> mlso, histology department
> hutt valley health
> lower hutt, new zealand
>
> ph. ++64 4 5709173
> fax ++64 4 5709214
> ___________________________________________________
>
>
>
<< Previous Message | Next Message >>