RE: dehydrated brain...

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From:"Bartlett, Jeanine" <>
To:'Pam Marcum' <>, a i d a n s c h u r r <>,
Content-Type:text/plain; charset="iso-8859-1"

We've used Ruffer's solution for restoring small, dried-out pieces, but I
don't know about on a whole brain!

Jeanine H. Bartlett, HT(ASCP)
Centers for Disease Control
1600 Clifton Rd., NE  MS/G-32
Atlanta, GA  30333

-----Original Message-----
From: Pam Marcum []
Sent: Tuesday, February 22, 2000 9:03 AM
To: a i d a n s c h u r r;
Subject: RE: dehydrated brain...

Unfortunately, once the complete dehydration is done there is very little
you can do.  If it was NBF there is even a good chance you have precipitated
buffer salts all over the outside as a crust.  This will further inhibit any
penetration of fluids.  Maybe someone else can add more.  Pam

-----Original Message-----
From: a i d a n s c h u r r []
Sent: Monday, February 21, 2000 11:10 PM
Subject: dehydrated brain...

Hi all,

An interesting question / problem...

We have a brain which was fixed approx. 2 years ago in NBF, then
stored.  Unfortunately the container was not sealed, and the NBF
evaporated.  We now have an extremely dehydrated and shrivelled
brain, and a consultant who wants histology on it!!  Any ideas as to
rehydration / salvage would be greatly appreciated.

Aidan Schurr, New Zealand

a i d a n   c   s c h u r r
     mlso,  histology department
      hutt valley health
       lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214

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