RE: Using CD31 for staining endothelial cells

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From:Abizar Lakdawalla <>
To:'Karen M Majahad' <>,
Content-Type:text/plain; charset="iso-8859-1"

Are you using the Pharmingen rat monoclonal. We just finished a staining
project for a client where we discovered that the way the tissue is fixed
has a lot to do with the staining quality. Pharmingen recommends a Zinc
based fixative which does give more effective staining results.

-----Original Message-----
From: Karen M Majahad []
Sent: Thursday, February 17, 2000 11:28 AM
Subject: Using CD31 for staining endothelial cells

Hello Histonetteers,
I am having problems with the immunostaining of CD31 on mouse
endothelial cells in paraffin sections. The antibody does not appear to
be staining all of the enothelial cells. I have tried using trypsin and
Vector Antigen unmasking solution. I have used several methods including
using both trypsin and antigen unmasking solution for pretreatment
methods. These sections are not tumors and have very new blood vessels
forming. Any information would be helpful.


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