RE: Steiner and Steiner Problem/Fungus Problem solved.

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From:"Abrahamson, Brian" <babraham@MACNEAL.COM>
To:"'Scott, Allison D'" <Allison_Scott@hchd.tmc.edu>, 'Histo Net' <histonet@pathology.swmed.edu>
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I have also experienced this problem using purchased controls for
spirochetes (Sigma) and running a Warthin Starry Stain.  The control is very
slow to turn while the patient is turned in the normal time.  This just
started happening recently and I thought perhaps it was because the moon was
in the wrong phase (ha).  Perhaps because it is a purchased control the
provider has inadvertently used something in the process that is inhibiting
the staining reaction.

Brian Abrahamson
Genesis Clinical Lab

	-----Original Message-----
	From:	Scott, Allison D [SMTP:Allison_Scott@hchd.tmc.edu]
	Sent:	Friday, February 25, 2000 4:13 PM
	To:	histonet@pathology.swmed.edu
	Subject:	Steiner and Steiner Problem/Fungus Problem solved.

	We are having a problem with the steiner and steiner.  After the
slides are
	put into the developer, the patient slides turn but the control
slide does
	not.  We originally thought that the hydroquinone was not any good,
so we
	got some new chemical.  The same thing happened again.We follow the
	procedure to the letter. IThe dry chemicals are not old.  Not that
it makes
	any difference.  We also have considered that the control slides may
not be
	any good also.  On Monday we will repeat the stain using another
control.
	By the way the controls are store bought. For those of you that
responded to
	my problem of fungus growing among us, the problem is with the
cytology
	area.  Were not sure where  in the cytology area but it is not with
anything
	that histology did.  If anyone has a solution to my steiner problem
please
	respond..
	Allison Scott  



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