RE: Sectioning araldite
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From: | "Smith, Allen" <asmith@mail.barry.edu> |
To: | 'Judi Lloyd' <lloyd@vet.upenn.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
Araldite is very hard: it needs to be softened to make it lie flat. Heat is
a good softener.
Put a drop of water on a clean glass slide. Use the end of a diagonally cut
wood splint to transfer the section (1/4 micron to 1 micron, no thicker) to
the water drop. Place the slide on a hotplate at about 80 degrees C (too
hot to leave your finger on it but not so hot that you can't touch it) until
the water evaporates completely.
-----Original Message-----
From: Judi Lloyd [mailto:lloyd@vet.upenn.edu]
Sent: Wednesday, February 16, 2000 1:47 PM
To: histonet@pathology.swmed.edu
Subject: Sectioning araldite
Hello Histonetters,
Since I usually am involved in bone pathology, I have a big
problem with a
project involving araldite sectioning. I have done some
Spurrs medium ages
ago but I cannot get the sections to lie flat on the slide
after cutting
with a glass knife. HELP. Any information would be helpful
as I am not an
EM tech. Cutting angles, slide preparation and staining
would help a lot.
I am just doing this as a screening for a lung problem to
see if the
problem actually exists. If the sections are positive, we
will probably
send then to a real EM lab, but since I have an
ultramicrotome, I have been
asked to do the screening. Thanks in advance for your
help. Judi Lloyd
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