RE: IHC staining and animal tissue

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From:Cynthia Favara <>
To:'mbpohl' <>,
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I cut a lot a mouse tissue and some of it is pretty old and dried out. I
soak blocks minimally in ice water nothing added, float for as long as
possible, use charged slides and dry overnight at room temperature. I have
steamed, autoclaved and microwaved sections will little or no loss. Are the
sections comming completely off the slides? In my hands greater than 0.3%
H202 can cause tissue destruction and the use of coverslips during staining
can also be a problem. Have also found that boiling during HIER is
detrimental. Hope this helps.
Cynthia Favara
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286

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