Mike,

We use AEC as chromogen for these cases.

Bryan

> ----------
> From: 	Mike or Carol Schreiber[SMTP:mjsch@pro-ns.net]
> Sent: 	February 16, 2000 3:38 PM
> To: 	HistoNet Server
> Subject: 	IHC and Melanin Pigment
> 
> Dear Histonetters,
>     How is anybody dealing with heavy deposits of Melanin pigment in
> (skin)
> tissue that is to be stained for HMB-45 and/or S-100? We are using DAKO's
> Envision+/DAB+ detection system, on the DAKO autostainer.  Will a Melanin
> Bleach procedure  using Potassium Permanganate and Oxalic Acid adversely
> affect subsequent immunostaining? I read something about using Azure-B as
> a
> counterstain instead of Hematoxylin, which is supposed to stain the
> melanin
> a green-blue thus differentiating it from the brown staining of the DAB
> chromogen. Has anybody tried this?  Thanks for any help you can give me
> with
> this problem.
> 
>     Mike Schreiber HT(ASCP)
>     Three Rivers Pathology
>     Roseville, Minnesota
> 
>