Hi Jennifer. I would try the iso-IHC procedure. There will be no
cross-reactivity problems. The method is essentially; (in a test tube)adding
a biotin label to your primary antibody by the use of a labeling reagent
(this reagent is specific to mouse monoclonal antibodies therefore
contaminating proteins do not interfere), excess labeling reagent is
adsorbed with a blocking reagent. The mixture is put on the slide,
incubated, washed, and a streptavidin-conjugate added. I don't think this
short description may make sense but I can send you more information if you
are interested.
Abizar
www.innogenex.com

-----Original Message-----
From: jennifer.hoover@pharma.Novartis.com
[mailto:jennifer.hoover@pharma.Novartis.com]
Sent: Monday, February 21, 2000 8:04 AM
To: Histonet@pathology.swmed.edu
Subject: Antibody Search!!!!!


     Hi to All!  I am currently in a dilemna as I am trying to stain
xenograft
tissue for mouse and rat CD45 positive cells.  Does anyone know of a
polyclonal
rabbit or goat anti rat or anti mouse CD45???  This would greatly improve
our
ability to confirm which cells are rat derived and which cells are mouse
derived
without the issue of x-reactivity which is currently a problem. I have
consulted
the Linscott Directory but with limited success.  Any suggestions would be
very
much appreciated!  Thank you in advance!


Jennifer Hoover
Novartis Pharmaceuticals