Pancreas In Situ

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From:Linda Prentice <>
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I'm passing on a request from a co-worker for help with his in-situ on
mouse pancreas.  I have been bragging about all the useful information that
I have aquired through the histonet, so if you are able to please help us
with this one.


>I am new to in situ hybridization and, with using DIG-labeled RNA, am
>able to obtain nice staining in various mouse tissue cryostat-cut
>sections.  Currently I'm trying to get this to work in an inflamed
>pancreas.  I haven't been very successful with pancreas and would
>like a protocol or reference for consistent, reproducible results on
>either mouse or human tissue that is either fresh frozen or
>   Thanks.

Linda Prentice
Department of Anatomy
University of California San Francisco
513 Parnassus Ave
Room S-1343
San Francisco CA 94143-0452
Phone 415-476-1439
Fax 415-476-4845

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