Optimisation help

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From:Emma Carter <E.Carter@OxfordBioMedica.co.uk>
To:'histonet' <histonet@pathology.swmed.edu>

My supervisor wants me to optimise 4 brand new antibodies, in thew shortest
possible time...

I am staining by hand, and am largely using cytospins/cell pellets or frozen
mouse tumour material.

Antibodies testing for (yeah right) are:

GFP, Human CD68, Hif 1alpha and P450....

Can _anyone_ help.....(short of coming here and doing it for me.....)

Any help would be sooooooo appreciated!


emma carter

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