Old cloudy formalin (was RE: FLT-1/FLK-1 ISH)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Pam Marcum <pmarcum@polysciences.com> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Wed, 16 Feb 2000, Pam Marcum wrote:
> Methanol is considered a stabilizer to slow the progression of formaldehyde
> back to paraformaldehyde by most companies. However, even stabilized you
> should watch 37 to 40% formaldehyde and if a cloud or precipitate appears in
> the solution it has polymerized back to paraformaldhdye. It is now
> primarily is simple terms formic acid. Do not use it.
The amount of formic acid in old cloudy formalin will not
significantly lower the concentration of formaldehyde and
its polymers, and the acidity is easily neutralized.
Formation of polymers is not associated with the Cannizzaro
reaction that generates methanol and formic acid in stored
formaldehyde solutions. Polymers can be depolymerized by
heating the suspension after addition of the buffer salts.
For more info consult the HistoNet Archives (www.histosearch.com)
which contain many messages and refs to books & papers. The
book "Formaldehyde" by Walker is excellent.
It is written in the literature (Stain Technol. 1940s; I don't
have the reference to hand) that heavily precipitated formalin
can be rejuvenated to a clear liquid by autoclaving it in small,
strong, tightly capped bottles. I have never tried this because
I didn't see the paper until about 1980, and since coming to
Canada in 1972 my lab has never been cold enough to polymerize
formalin to a solid. Back in Blighty (1960s), where they
turned off the heating at night, precipitates in the formalin
were part of the winter scene, and nobody needed to ask why
conc. acetic acid was called "glacial."
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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