Negative Reagent Controls For Immuno

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From:"Mackinnon, John" <>
To:"HistoNet (E-mail)" <>
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When running negative reagent controls you should be running a negative for
each block, if you are running both monoclonal and polyclonal antibodies you
should run a negative for each of these.  If one of your antibodies requires
HIER another enzyme and one no pretreatment you should be running a negative
for each of these.  All of your controls must be treated the same way that
your test sections are treated, if you don't do this you are not really
controlling the procedure.  Expense is no excuse for not running proper QC.
I can't remember ever hearing a biochemist saying that they were not going
to run all of the appropriate QC because it seemed excessive and expensive.

John MacKinnon MLT, ART
Senior Technologist, Pathology
Lakeridge Health Oshawa
Ontario, Canada

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