Hi, i'm an honours student at the Univerty of Melbourne/Australia and am having trouble finding information about fixing samples as I will be working on foreskin and WHOLE penis samples & wanting to do IHC  If anyone could help with this & the following problems I would be most appreciative:
1. How soon after removal from the patient do foreskins and penises have to be frozen (& what procedure would I utilize) so I avoid cell damage and denaturation? (as the only source of specimens is from the coroner)??
2. Is it possible to cryo a whole penis and get uniform freezing because most penises are a bit larger than the recommended cubic centimetre sample size? 
3. Is there a better fixing method in which cell surface Ag's are still preserved so we can still stain specifically for them and maybe fix entire foreskins and penises (eg, NBF)?
4. How long can cryo sections be stored & @ what temp??                              Cheers & THANKS in advance for ANY suggestions....Scott. 

--
BRUCE ABALOZ
HISTOLOGIST		      
DEPARTMENT of ZOOLOGY	         *  ph:    +61 3 93446282
The UNIVERSITY of MELBOURNE      *  fax:   +61 3 93447909 
Parkville Victoria.3052          *  email: b.abaloz@zoology.unimelb.edu.au                                                                       
AUSTRALIA.
                    IF YOU WANT TO BE HAPPY......BE!!
                    I DO NOT SEEK............I FIND!!

This message is confidential;its contents do not constitute a commitment by The University Of Melbourne except where provided for in a written agreement between you and The University Of Melbourne. Any unauthorised disclosure, use or dissemination, either whole or partial, is prohibited. If you are not the intended recipient of the message, please notify the sender immediately.