Histone H3

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From:"Boucher, Germaine G" <germaine_g_boucher@groton.pfizer.com>
To:"'HistoNet@Pathology.swmed.edu'" <HistoNet@Pathology.swmed.edu>
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Hello Histonetters,
Has anyone used DAKO's kit for in situ hybridization of histone h3?  I have
been using this kit and have been getting inconsistent results.  I have also
been having quite a problem with precipitation of the BCIP chromogen.  Any
thoughts?  At this point I'm getting really frustrated...


Germaine Boucher
Pathology EM Lab
Pfizer Central Research, Groton, CT

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