Hale's colloidal iron

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From:"Smith, Allen" <asmith@mail.barry.edu>
To:"'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
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From A.G.E. Pearse, Histochemistry, Theoretical and Applied, 3rd edition,
1968, Williams & Wilkins, Baltimore, p. 670.
"Unfixed fresh frozen sections ...are excellent. Carnoy or formalin-fixed
paraffin sections are usually recommended.
Sections should be mounted on slides without any adhesive.
1.	Bring sections to water...
2.	Flood with dialysed iron (1 volume dialysed iron, British Drug
Houses, Ltd., Poole, England, plus 1 volume 2 M acetic acid) for 10 minutes.
3.	Wash well with distilled water.
4.	Flood with acidic ferrocyanide solution (1 volume 0.02 M potassium
ferrocyanide plus 1 volume 0.14 M hydrochloric acid), leave for 10 minutes.
5.	Wash in water.
6.	Counterstain paraffin sections with ... 1% aqueous neutral red (1
minute).  Counterstaining not recommended for frozen sections.
7.	Dehydrate in alcohol, clear in xylene and mount."

Pearse also gives a recipe for making your own dialysed iron.  Believe me:
you don't want to!
			Allen A. Smith
			School of Graduate Medical Sciences
			Barry University
			Miami Shores, FL 33161

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