Hi,
I was wondering if anyone had any special techniques for frozen
sections.  Liver tissue tends of curl up after being put in the
Oil Red O solution.  We cut them at 10 microns.  If we cut them
at 8 microns, we usually do not have the problem.
We use tissue that has been fixed in 10% formalin, then rinsed
and put in sucrose solution.
Our pathologists like the sections to be cut at 10 microns.

Any suggestions would be appreciated.

Thanks,

Sharon