Hello Histonetters,

I hope someone can help me. Does anyone know how to
fix or otherwise harden gelatin without the use of
glutaraldehyde?
I am currently producing tripple immunofluorescence
from slices of brain tissue. In order to get thin
enough (50-60micron) slices the tissue is embedded in
gelatin and cut using a vibratome. If immuno is not
needed this technique works beautifully when blocks
are fixed in glut containing fixative. However,
glutaraldehyde destroys immunoreactivity and without
it the blocks are too soft and difficult to cut, so I
need an alternative.

Any Ideas?
Thanks in advance.

Peter Bannister.

=====
Peter Bannister
Senior Research technician
UCL Med. School
London.
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com