orientation of small tissues with lumens

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
To:histonet@pathology.swmed.edu
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If the problem persists, embed the vessels so they lay on their sides,
then, let block harden, pop it out and re embed the block so the vessets stand
on end to see lumen.

This can be done with paraffin and frozen tissues, had to do this on 
occasion, more as a tissue orientation correction, but it works, and saves
frustration of getting the vessels on end in the beginning!!

Works with GMA embedded tissues also. Basically a double embedding, extra
paraffin can be carefully trimmed away, but a deeper mold helps if the tissues
are a bit longer than depth of the mold.  

Hope this helps,

Gayle Callis





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