mast cells on frozens

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From:Rebecca S Smith <>
To:"HistoNet Server" <>
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I used acetone.  All my staining with a 4 hour exposure to 0.5 pH 0.5% 
Toluidine Blue in lungs on sheep and cattle were negative.  In fixed 
tissue, as reported prior to this in many papers, Carnoy's is the best 
fixative.  I am planning on recutting my frozens and trying several 
different fixatives.  Just thought I'd get Histonet's input before my final 
decision on  which one's might be best to try.
At 12:21 AM 2/6/2000 -0600, you wrote:

>Date: 5 Feb 2000 12:50:04 -0600
>From: Gayle Callis <>
>Subject: mast cells on frozens
>What fixative are you using?
>Are you staining for connective tissue mast cells or mucosal mast cells found
>in gut?
>What stain are you trying to do for the mast cells?
>Just curious
>Gayle Callis

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