mast cells on frozens
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From: | Rebecca S Smith <bssvpisu@iastate.edu> |
To: | "HistoNet Server" <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii"; format=flowed |
I used acetone. All my staining with a 4 hour exposure to 0.5 pH 0.5%
Toluidine Blue in lungs on sheep and cattle were negative. In fixed
tissue, as reported prior to this in many papers, Carnoy's is the best
fixative. I am planning on recutting my frozens and trying several
different fixatives. Just thought I'd get Histonet's input before my final
decision on which one's might be best to try.
At 12:21 AM 2/6/2000 -0600, you wrote:
>Date: 5 Feb 2000 12:50:04 -0600
>From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
>Subject: mast cells on frozens
>
>What fixative are you using?
>
>Are you staining for connective tissue mast cells or mucosal mast cells found
>in gut?
>
>What stain are you trying to do for the mast cells?
>
>Just curious
>
>Gayle Callis
>
>
>----------------------------------------------------------------------
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