frozen sections

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From:Atoska Gentry <>
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does anyone know what may be causing my frozen mouse brain sections to
become detached from the gum tragcanth cork mounting upon placing them in
an ultralow freezer for holding/storage ?  We freeze our tissue samples
mounted on the cork with the gum tragcanth and immerse in isopentane
chilled by liquid nitrogen for 12 sec. and then we store them at -80C until
sectioning.  Up until this point I haven't had any problems.  The majority
of our tissuses have been muscle ( cat/dog)  and for some reason these
mouse tissues are driving me crazy especially the brain.  Any and all help
will be much appreciated.  Thanks,  Atoska

Atoska S. Gentry B.S., HT(ASCP)
Research Assistant
Scott-Rtichey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
PH (334) 844-5579
FAX (334) 844-5850

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