frozen mast cells

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From:Rebecca S Smith <>
To:The Histonet <>
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I have been performing a study with one of our research pathologists on 
Mast cells.  We have done paraffin sectioning and now he want to do 
frozens.  I did the first batch with our routine fixative for frozens: 
Acetone.  There was virtually no staining what-so-ever.  Can anyone 
comments on what a good fixative for the preservation of mast cells in 
frozen sections might be?

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