frozen fixation
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From: | Rebecca S Smith <bssvpisu@iastate.edu> |
To: | "HistoNet Server" <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii"; format=flowed |
My experience with acetone on spleen is, it doesn't work very well. The
reason being the extremely bloody nature of the tissue. It tends to bubble
many different fixatives. I would think that an alcoholic fixative would
be better for the architecture.
At 09:10 AM 2/4/2000 -0600, you wrote:
>ate: 3 Feb 2000 14:42:06 -0600
>From: jennifer.hoover@pharma.Novartis.com
>Subject: Acetone Fixation??
>
> Hi to All! I just have a question concerning Acetone fixation of frozen
>tissue, especially concerning spleen. Could you please share your protocols
>and
>other special tricks of the trade? Currently whether I fix at 4C or -20C for
>3
>or 5 minutes the Acetone seems to almost chew up the spleen on the slide! I
>have not encountered this problem in the past with other tissue types. Any
>suggestions would be very much appreciated. Thank you for sharing the
>knowledge!
>
>
>Jennifer Hoover
>Novartis Pharmaceuticals
>
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