frozen fixation

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From:Rebecca S Smith <>
To:"HistoNet Server" <>
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My experience with acetone on spleen is, it doesn't work very well.  The 
reason being the extremely bloody nature of the tissue.  It tends to bubble 
many different fixatives.  I would think that an alcoholic fixative would 
be better for the architecture.

At 09:10 AM 2/4/2000 -0600, you wrote:
>ate: 3 Feb 2000 14:42:06 -0600
>Subject: Acetone Fixation??
>      Hi to All!  I just have a question concerning Acetone fixation of frozen
>tissue, especially concerning spleen.  Could you please share your protocols
>other special tricks of the trade?  Currently whether I fix at 4C or -20C for
>or 5 minutes the Acetone seems to almost chew up the spleen on the slide!  I
>have not encountered this problem in the past with other tissue types.  Any
>suggestions would be very much appreciated.  Thank you for sharing the
>Jennifer Hoover
>Novartis Pharmaceuticals

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