freezing of marine larvae for crystat
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From: | "Keith Ryan" <KPR@wpo.nerc.ac.uk> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hello Histoland
I have someone here who wants to freeze and cut sections of marine
larvae as thinly as possible (5 microns?) for immuno. The specimens
are 100-200 microns in length.
1. What would be the best way of handling these small items?
2. What would be the best support/medium e.g TissueTek?
3. What about cryoprotection? I am familiar with 2.3 molar sucrose
for EM.
4. Any general tips for immuno (protein/amino peptidases)?
Best wishes - Keith (again!)
_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England
Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102
e-mail: kpr@wpo.nerc.ac.uk
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